Lab location
Our lab is located in Louvain-la-Neuve, around 30 km in the South of Brussels.
Equipments
The nBio lab (Alsteens & Dufrêne teams) is equipped with state-of-the art AFMs to investigate single-molecule intearctions at high-resolution on purified receptors or combined with an inverted epifluoresence microscope to study biological process directly on living cells.
Three inverted optical microscopes with epifluorescence are available with 10x, 20x, 40x, 63x & 100x objectives. All are combined with the above-mentionned AFMs and placed in thermo regulated chamber.
The new LSM980 with Airyscan2.0 and Fast scan allows gentle superresolution imaging and high speed. The resolution is around 120 nm (in x,y) and 350 nm (in z) and teh acquisition speeds is 27fps (at 480 x 480 pixels). The confocal is equipped with 20x, 40x (1.2 NA) and 63x (1.2 NA) objectives and 5 lasers (405, 458, 488, 514 & 633). The confocal is combined with Bruker Resolve and set up in a thermo regulated chamber.
The lab is equipped with various other techniques enabling the imaging and quantification of protein, nucleic acids and cells. The Thyphoon is equipped with 4 lasers (2 in the NIR and 2 in the Vis) supports multiple imaging modes including fluorescence red, gree, blue and NIR. The Imager 600 enables sensitive and quality imaging of DNA and protein gels and chemiluminescence in Westen blot. The Bio-Rad S3e cell sorter is an automated cell sorter equipped with two lasers (488 and 561 nm)
Amersham Typhonn | Amersham Imager | Bio-Rad S3e |
To have access to these machines, please contact Kimberley Durlet. To reserve one of these instruments and/or check the schedule, please follow this Link
© David Alsteens. All rights reserved. | Photos by NanoBioPhysics lab | Design by TEMPLATED.